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When a body is dissected, its structures are cut apart in order to observe their physical attributes and their relationships to one another. Dissection is still used in medical schools, anatomy courses, and in pathology labs. In order to observe structures in living people, however, a number of imaging techniques have been developed. These techniques allow clinicians to visualize structures inside the living body such as a cancerous tumor or a fractured bone.

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From Vesalius’s exact descriptions of the skeleton, muscles, blood vessels, nervous system, and digestive tract, his successors in Padua progressed to studies of the digestive glands and the urinary and reproductive systems. Hieronymus Fabricius, Gabriello Fallopius, and Bartolomeo Eustachio were among the most important Italian anatomists, and their detailed studies led to fundamental progress in the related field of physiology. William Harvey’s discovery of the circulation of the blood, for instance, was based partly on Fabricius’s detailed descriptions of the venous valves.

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The use of the microscope in discovering minute, previously unknown features was pursued on a more systematic basis in the 18th century, but progress tended to be slow until technical improvements in the compound microscope itself, beginning in the 1830s with the gradual development of achromatic lenses, greatly increased that instrument’s resolving power. These technical advances enabled Matthias Jakob Schleiden and Theodor Schwann to recognize in 1838–39 that the cell is the fundamental unit of organization in all living things.

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The skeleton also protects several vital organs such as the heart, lungs and the liver. Bones are attached to other bones through ligaments, a fibrous connective tissue.

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In contrast, micro- means “small,” and microscopic anatomy is the study of structures that can be observed only with the use of a microscope or other magnification devices (Figure 1b). Microscopic anatomy includes cytology, the study of cells and histology, the study of tissues. As the technology of microscopes has advanced, anatomists have been able to observe smaller and smaller structures of the body, from slices of large structures like the heart, to the three-dimensional structures of large molecules in the body.

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Whereas anatomy is about structure, physiology is about function. Human physiology is the scientific study of the chemistry and physics of the structures of the body and the ways in which they work together to support the functions of life. Much of the study of physiology centers on the body’s tendency toward homeostasis. Homeostasis is the state of steady internal conditions maintained by living things. The study of physiology certainly includes observation, both with the naked eye and with microscopes, as well as manipulations and measurements. However, current advances in physiology usually depend on carefully designed laboratory experiments that reveal the functions of the many structures and chemical compounds that make up the human body.

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